Review



mouse anti-post synaptic density protein 95 (psd-95)  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Novus Biologicals mouse anti-post synaptic density protein 95 (psd-95)
    Mouse Anti Post Synaptic Density Protein 95 (Psd 95), supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/mouse+anti+psd+95/pmc07801286-87-34-42
    Average 90 stars, based on 1 article reviews
    mouse anti-post synaptic density protein 95 (psd-95) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke
    Article Snippet: The primary antibodies used in this study were mouse antineuronal nuclei (NeuN, 1:1000, Chemicon, Burlington, MA, USA, cat. no. mab377), rabbit anti-microtubule associated protein 2 (MAP2, 1:600, Millipore, Burlington, MA, USA, cat. no. ab5622), mouse anti-post synaptic density protein 95 (PSD-95, 1:500, Novus, Saint Charles, MO, USA, cat. no. nbp2-12872), goat anti-ionized calcium-binding adapter molecule 1 (Iba-1, 1:500, Novus, Saint Charles, MO, USA, cat. no. nb100-1028), rabbit anti-purinergic receptor P2Y (P2RY12, 1:500, Brigham Health, Boston, MA, USA), rat anti-CD68 (1:500, Biorad, Hercules, CA, USA), rabbit anti-interleukin 4 (IL-4, 1:300, Thermo fisher Scientific, Waltham, MA, USA, cat. no. pa5-25165), rabbit anti-nitric oxide synthase 2 (NOS2, 1:300, rabbit, Millipore, Burlington, MA, USA, cat. no. ab5382), mouse anti-2',3'-Cyclic-nucleotide 3'- phosphodiesterase (CNPase, 1:300, Abcam, Cambridge, UK, cat. no. ab6319), rabbit anti-myelin basic protein (MBP, 1:500, Abcam, Cambridge, UK, cat. no. ab40390) and mouse anti-glial fibrillary acidic protein (GFAP, 1:500, Sigma, St. Louis, MO, USA, cat. no. g3893).



    Similar Products

    monoclonal mouse anti-post synaptic density protein 95 (psd-95  (Millipore)
    90
    Millipore monoclonal mouse anti-post synaptic density protein 95 (psd-95
    Monoclonal Mouse Anti Post Synaptic Density Protein 95 (Psd 95, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/psd+95+antibody/pmc03672166-76-20-29
    Average 90 stars, based on 1 article reviews
    monoclonal mouse anti-post synaptic density protein 95 (psd-95 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse anti-post synaptic density 95 protein (psd95) 1:20000 1h  (Millipore)
    90
    Millipore mouse anti-post synaptic density 95 protein (psd95) 1:20000 1h
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density 95 Protein (Psd95) 1:20000 1h, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/psd+95+antibody/pmc03686744-64-10-19
    Average 90 stars, based on 1 article reviews
    mouse anti-post synaptic density 95 protein (psd95) 1:20000 1h - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    monclonal mouse anti-post synaptic density protein 95 igg2a  (Millipore)
    90
    Millipore monclonal mouse anti-post synaptic density protein 95 igg2a
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Monclonal Mouse Anti Post Synaptic Density Protein 95 Igg2a, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/horseradish+peroxidase/pm34933788-127-30-40
    Average 90 stars, based on 1 article reviews
    monclonal mouse anti-post synaptic density protein 95 igg2a - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse anti psd95 antibody  (Bioss)
    92
    Bioss mouse anti psd95 antibody
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Psd95 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/Post-synaptic+density+protein+95+Antibody/pm34133789-52-0-12
    Average 92 stars, based on 1 article reviews
    mouse anti psd95 antibody - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier
    mouse anti-post synaptic density protein 95 clone 7e3–1b8  (Millipore)
    90
    Millipore mouse anti-post synaptic density protein 95 clone 7e3–1b8
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density Protein 95 Clone 7e3–1b8, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/monoclonal+primary+antibody+clone+7e3+1b8/pm34481832-96-44-55
    Average 90 stars, based on 1 article reviews
    mouse anti-post synaptic density protein 95 clone 7e3–1b8 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse anti-post synaptic density protein 95 (psd95) antibody #mab1596  (Millipore)
    90
    Millipore mouse anti-post synaptic density protein 95 (psd95) antibody #mab1596
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density Protein 95 (Psd95) Antibody #Mab1596, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/psd+95+antibody/pm30585381-101-10-18
    Average 90 stars, based on 1 article reviews
    mouse anti-post synaptic density protein 95 (psd95) antibody #mab1596 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse anti-post synaptic density protein 95 (psd-95)  (Novus Biologicals)
    90
    Novus Biologicals mouse anti-post synaptic density protein 95 (psd-95)
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density Protein 95 (Psd 95), supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/mouse+anti+psd+95/pmc07801286-87-34-42
    Average 90 stars, based on 1 article reviews
    mouse anti-post synaptic density protein 95 (psd-95) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse anti post synaptic density protein 95  (Novus Biologicals)
    93
    Novus Biologicals mouse anti post synaptic density protein 95
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density Protein 95, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/PSD-95+Antibody+(7E3)/10__14336_slash_ad__2020__0406-125-34-42
    Average 93 stars, based on 1 article reviews
    mouse anti post synaptic density protein 95 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mouse anti-post synaptic density protein-95 (psd95)  (Millipore)
    90
    Millipore mouse anti-post synaptic density protein-95 (psd95)
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density Protein 95 (Psd95), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/psd+95+antibody/pm30841933-46-0-8
    Average 90 stars, based on 1 article reviews
    mouse anti-post synaptic density protein-95 (psd95) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse anti-post-synaptic density protein 95 psd95  (Millipore)
    90
    Millipore mouse anti-post-synaptic density protein 95 psd95
    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein <t>(PSD95)</t> and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.
    Mouse Anti Post Synaptic Density Protein 95 Psd95, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+post+synaptic+density+protein+95/psd+95+antibody/pmc05581809-96-22-31
    Average 90 stars, based on 1 article reviews
    mouse anti-post-synaptic density protein 95 psd95 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein (PSD95) and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.

    Journal: PLoS ONE

    Article Title: Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry

    doi: 10.1371/journal.pone.0068063

    Figure Lengend Snippet: Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein (PSD95) and Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B ) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) and TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C – E ) The high magnification of the inset in B is reproduced in different merge images and by the relative split channels. C ) Merge image of Cb and V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D ) Merge image of TA and Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E ) Merge image of TA and V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F ) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) and TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm.

    Article Snippet: 131011), rabbit anti-Synapsin-I 1:1000 for 1 ON (Novus Biologicals NB300-104), mouse anti-post synaptic density 95 protein (PSD95) 1:20000 1h (Millipore, cod.

    Techniques: Western Blot, Staining, Expressing